Search results for "Polyphasic characterization"

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Clonal populations of Clavibacter michiganensis subsp michiganensis are responsible for the outbreaks of bacterial canker in greenhouse tomatoes in I…

2015

Clavibacter michiganensis subsp. michiganensis (Cmm) strains, collected in greenhouses from 17 farms during tomato bacterial canker outbreaks occurring between 2005 and 2008 in Sicily, were analysed by a multiphasic approach. Population studies were conducted to investigate the possible sources of inocula. Cmm strains were characterized by PCR assays targeting virulence genes, fingerprinting techniques, metabolic profiles and virulence. These strains were comparatively analysed with Cmm strains isolated in other parts of Italy over a period of 15 years. Chromosomal genes encoding virulence determinants tomA, ppaA, chpC, and the plasmid-encoded genes pat-1 and celA were detected by PCR in al…

0106 biological sciences0301 basic medicineBacterial cankerBOX-PCR; Clavibacter michiganensis subsp; michiganensis; fAFLP; polyphasic characterization; tomato bacterial wilt and cankerClavibacter michiganensis subsp030106 microbiologyPopulationVirulencePlant ScienceHorticultureBiologySubspeciesfAFLP01 natural sciencesPolyphasic characterizationMicrobiologyClavibacter michiganensis subsp. michiganensi03 medical and health sciencesmichiganensisGeneticseducationGeneBOX-PCReducation.field_of_studyHaplotypeOutbreakSettore AGR/12 - Patologia VegetaleTomato bacterial wilt and cankerbiology.organism_classificationAgronomy and Crop ScienceClavibacter michiganensis010606 plant biology & botany

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